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Amino acid sequence of the rabies virus glycoprotein deduced from its cloned gene
Abstract:
Double-stranded complementary DNA was synthesized from rabies virus-specific glycoprotein mRNA and inserted into the Pst I site of pBR322. The glycoprotein inserted sequence contains approximately 1.75 kilobase pairs and lacks only approximately 35 nucleotides from the 5' terminus of the glycoprotein mRNA. The nucleotide sequence indicates a polypeptide of 524 amino acids beginning with an initiation codon ATG and ending with a termination of codon TGA. The first 19 amino acids make up a signal peptide preceding the sequence lys-phe-pro-ile-tyr-thr- which has been identified by the N-terminal analysis of amino acids of the purified rabies virus glycoprotein.
Insights
Researchers synthesized complementary DNA from rabies virus glycoprotein mRNA, creating a sequence encoding a 524-amino acid protein. This DNA sequence provides insights into rabies virus glycoprotein structure and function.
Area of Science:
- Molecular Biology
- Virology
- Genetics
Background:
- Rabies virus glycoprotein is crucial for viral entry and immune response.
- Understanding the glycoprotein's genetic makeup is essential for vaccine development and antiviral strategies.
Purpose of the Study:
- To synthesize and characterize the complementary DNA (cDNA) encoding the rabies virus glycoprotein.
- To determine the nucleotide sequence and predict the amino acid sequence of the glycoprotein.
Main Methods:
- Complementary DNA synthesis from rabies virus glycoprotein mRNA.
- Insertion of cDNA into the pBR322 plasmid vector.
- Nucleotide sequencing of the inserted DNA fragment.
Main Results:
- A 1.75 kilobase pair DNA sequence was obtained, closely matching the glycoprotein mRNA.
- The sequence encodes a polypeptide of 524 amino acids, including a signal peptide.
- N-terminal amino acid analysis confirmed the predicted signal peptide sequence.
Conclusions:
- The synthesized cDNA represents a significant portion of the rabies virus glycoprotein mRNA.
- The determined nucleotide and amino acid sequences provide a basis for further functional studies of the glycoprotein.