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Updated: Aug 19, 2026

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
Purification of tritium-labeled cholera toxin
Abstract:
Cholera toxin was labeled with tritium by the Wilzbach technique, and highly purified radiolabeled toxin was obtained by Sephadex column chromatography and disc gel electrophoresis. 3H-labeled cholera toxin retained its biological activity and chemical stability and had a specific activity of 405.9 muCi/mumol. The methods utilized in extraction and purification of 3H-labeled toxin may be advantageous for preparation of other biologically active radiolabeled proteins.
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