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Serum chromatographic pattern in different dialysis strategies.
The International Journal of Artificial Organs
|September 1, 1978
Summary
Different hemodialysis strategies effectively remove medium-sized molecules, halving serum levels. Sequential ultrafiltration also captures these molecules, but hemoperfusion capacity is limited.
Area of Science:
- Nephrology
- Biomedical Engineering
- Clinical Chemistry
Background:
- Dialysis strategies aim to remove uremic toxins, including medium-sized molecules.
- Evaluating the efficacy of different dialyzers and hemoperfusion is crucial for optimizing patient outcomes.
- Understanding molecule removal dynamics across various dialysis techniques is essential.
Purpose of the Study:
- To compare the removal of medium-sized molecules using different hemodialysis strategies.
- To assess the effectiveness of sequential ultrafiltration and hemoperfusion in toxin clearance.
- To investigate the impact of dialyzer type and system configuration on molecule removal.
Main Methods:
- Serum samples were analyzed using Sephadex G 15 chromatography.
- Hemodialysis was performed using Gambro 1.5 m2, RP6 1 m2, and Vita 2 1.2 m2 dialyzers.
- Sequential ultrafiltration with RP 6 and hemoperfusion with Absorba 300 C were employed.
Main Results:
- No significant difference in medium-sized molecule removal was observed between the tested dialyzers.
- Dialysis generally reduced serum levels of medium-sized molecules by approximately 50%.
- A substantial amount of medium-sized molecules was found in the ultrafiltration fluid, indicating efficient capture during this phase.
Conclusions:
- Hemodialysis efficacy for medium-sized molecule removal is comparable across the studied dialyzers.
- Sequential ultrafiltration is an effective method for removing medium-sized molecules.
- The hemoperfusion device (Absorba 300 C) showed limited capacity for medium-sized molecules, becoming saturated within one hour.