Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Control of hemoglobin expression in chick embryonic development.

L S Haigh, S Hellewell, I B Roninson

    Progress in Clinical and Biological Research
    |January 1, 1982
    PubMed
    Summary

    DNA methylation patterns in chick globin genes reveal tissue-specific regulation. Specific sites within the alpha-globin gene cluster show demethylation in erythroid cells, correlating with gene expression, while sperm and brain cells exhibit high methylation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genome Editing As an Approach to the Study of in Vivo Transcription Reprogramming.

    Doklady. Biochemistry and biophysics·2020
    Same author

    Beta dimethylaminoethyl benzhydryl ether hydrochloride (benadryl) in treatment of urticaria.

    Archives of dermatology and syphilology·2010
    Same author

    Cellular senescence induced by aberrant MAD2 levels impacts on paclitaxel responsiveness in vitro.

    British journal of cancer·2009
    Same author

    ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : I. Site of Maturation of Erythrocytes in Rana catesbeiana.

    The Journal of cell biology·2009
    Same author

    ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana.

    The Journal of cell biology·2009
    Same author

    ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : III. Immunochemical Detection of Tadpole and Frog Hemoglobins (Rana catesbeiana) in Single Erythrocytes.

    The Journal of cell biology·2009

    Area of Science:

    • Genetics
    • Molecular Biology
    • Epigenetics

    Background:

    • DNA methylation is a key epigenetic mechanism regulating gene expression.
    • The alpha-globin gene cluster plays a crucial role in erythropoiesis.
    • Tissue-specific DNA methylation patterns are essential for cellular differentiation.

    Purpose of the Study:

    • To investigate DNA methylation patterns at specific sites of the chick alpha-globin gene cluster.
    • To correlate methylation status with gene expression in different cell types.
    • To identify potential regulatory elements associated with the alpha-globin cluster.

    Main Methods:

    • Analysis of cytosine methylation (5-MeCpG) at specific sites within the chick alpha-globin gene cluster.
    • Comparison of methylation patterns in DNA from embryonic and adult erythroid cells, brain cells, and sperm cells.

    Related Experiment Videos

  • Southern blot analysis or similar techniques to detect methylation.

    • Main Results:

    • Sperm DNA showed complete methylation at the investigated sites.
    • Brain DNA exhibited methylation with some exceptions.
    • Erythroid cells displayed no or undermethylation at sites within or near actively expressed embryonic or adult alpha-globin genes, respectively.
    • Other non-gene-associated sites did not consistently follow the predicted methylation patterns.
    • A distinct cluster of 5-methylcytosine (5-MeCpC) methylation sites was identified 5' to the alpha-globin cluster in all examined tissues.

    Conclusions:

    • DNA methylation status at the chick alpha-globin gene cluster is tissue-specific and correlates with gene expression in erythroid cells.
    • The findings support a model where demethylation of specific sites is linked to gene activation.
    • Additional regulatory elements, such as the identified 5-MeCpC cluster, may influence alpha-globin gene regulation.