[Oriented migration of rat polymorphonucleocytes induced by 4-hydroxynonenal and...

The influence of the preincubation with 4-hydroxy-2,3-trans-nonenal (HNE) or formylmethionyl-leucyl-phenylalanine (fMLP) on the oriented migration of rat polymorphonuclear leukocytes (PMN) stimulated by fMLP or HNE, respectively, was investigated. Further, the chemotactic effect of the two agents assayed together at doses deprived alone of chemotactic power, was determined. Our results show that the preventive contact of PMN with HNE does not affect the ability of the leukocytes to respond chemotactically to fMLP. Similarly, the cell preincubation with fMLP is unable to influence the HNE chemotactic power. Doses of HNE or fMLP that are not chemotactic alone, stimulate significantly the PMN oriented migration when assayed together.