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Evolutionary conservation of vitellogenin genes.

T C James, U M Bond, C A Maack

    DNA (Mary Ann Liebert, Inc.)
    |January 1, 1982
    PubMed
    Summary

    Vitellogenin genes in insects, amphibians, and birds show remarkable evolutionary conservation. DNA hybridization studies reveal shared sequences, indicating ancient gene origins across diverse oviparous species.

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    Insect molecular biology·2011

    Area of Science:

    • Molecular Biology
    • Evolutionary Biology
    • Genetics

    Background:

    • Vitellogenins are crucial yolk proteins synthesized in oviparous (egg-laying) animals.
    • Understanding the evolutionary conservation of vitellogenin genes provides insights into reproductive biology across species.

    Purpose of the Study:

    • To investigate the evolutionary conservation of vitellogenin genes across insects, amphibians, and birds.
    • To determine the extent of sequence homology in vitellogenin genes between different oviparous species.

    Main Methods:

    • Solution hybridization kinetics and thermal stability analysis.
    • Southern blot analysis of restriction endonuclease-digested genomic DNA.
    • Hybridization using complementary DNA (cDNA) probes derived from vitellogenin mRNA.

    Main Results:

    • High degree of conservation observed in vitellogenin coding sequences among Xenopus, chicken, and migratory locust.
    • cDNA probes hybridized significantly to vertebrate DNA but not bacterial DNA.
    • Southern blot analysis confirmed homology of vitellogenin gene sequences across diverse vertebrates and invertebrates.
    • Repetitive DNA sequences related to Xenopus vitellogenin cDNA found in locust genome.

    Conclusions:

    • Vitellogenin genes exhibit remarkable evolutionary conservation across insects, amphibians, and avian species.
    • Evidence suggests ancient origins and shared ancestry for vitellogenin genes in oviparous animals.
    • The findings highlight the conserved role of vitellogenins in reproduction across a wide phylogenetic range.

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