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Identification of Protein Interacting Partners Using Tandem Affinity Purification
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Published on: February 25, 2012

The rabbit properdin system: I. Identification of a new factor and purification of rabbit properdin

G B Naff, S W Hedrick, W D Ratnoff

    Journal of Immunology (Baltimore, Md. : 1950)
    |June 1, 1980
    PubMed

    Pure preparations of rabbit properdin were obtained from rabbit serum by ion-exchange chromatography. These preparations functioned as properdin when they were measured with the zymosan assay or with a serum reagent selectively depleted of properdin by a specific immunoabsorbent. Properdin in these preparations was in its activated state. A new serum factor was required to measure properdin activity when purified preparations of rabbit properdin were tested with the zymosan assay. This factor was designated as ZBP, or zymosan-binding protein. ZBP appeared to be distinct from known components of the alternative complement pathway and the classical complement system, and it did not appear to be an immunoglobulin.

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