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Related Experiment Videos

Properdin factor B (Bf) as an exclusion determinate in parentage testing.

D Dykes, H Polesky

    Human Heredity
    |January 1, 1980
    PubMed
    Summary

    Properdin factor B (Bf) phenotyping in paternity cases revealed specific gene frequencies in Minnesota Whites. This analysis helps determine paternity with a calculated exclusion probability of 0.1442.

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    Area of Science:

    • Forensic genetics
    • Immunogenetics

    Background:

    • Properdin factor B (Bf) is a component of the alternative pathway of complement activation.
    • Genetic polymorphisms in Bf are valuable for forensic and paternity testing.

    Purpose of the Study:

    • To determine the gene frequencies of Properdin factor B (Bf) in a Minnesota White population.
    • To assess the utility of Bf in paternity testing by calculating exclusion probabilities.
    • To describe a method for simultaneous electrophoresis of Bf and Gc proteins.

    Main Methods:

    • Phenotyping of Properdin factor B (Bf) was performed on 519 individuals involved in paternity cases.
    • Gene frequencies for Bf alleles (BfF, BfF1, BfS, BfSO.7) were calculated.
    • Exclusion probabilities were determined based on observed gene frequencies.
    • Simultaneous electrophoresis technique for Bf and Gc proteins was developed.

    Main Results:

    • Observed gene frequencies in Minnesota Whites: BfF = 0.1870, BfF1 = 0.0105, BfS = 0.7985, BfSO.7 = 0.0040.
    • The calculated exclusion probability for Bf was 0.1442.
    • Bf contributed to 15% of the 88 exclusions observed in the paternity cases.
    • A method for simultaneous electrophoresis of Bf and Gc was successfully described.

    Conclusions:

    • Properdin factor B (Bf) genetic polymorphisms are useful in paternity testing.
    • The established gene frequencies provide a basis for forensic and paternity investigations in this population.
    • The simultaneous electrophoresis technique offers an efficient method for analyzing both Bf and Gc markers.

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