Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon towards...
Elastin is Responsible for Tissue Elasticity01:12

Elastin is Responsible for Tissue Elasticity

Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Latent-transforming growth factor beta-binding protein-2 (LTBP-2) is required for longevity but not for development of zonular fibers.

Matrix biology : journal of the International Society for Matrix Biology·2020
Same author

Macrophage secretion of miR-106b-5p causes renin-dependent hypertension.

Nature communications·2020
Same author

Contribution of metabolic disease to bone fragility in MAGP1-deficient mice.

Matrix biology : journal of the International Society for Matrix Biology·2018
Same author

Loss of Elastic Fiber Integrity Compromises Common Carotid Artery Function: Implications for Vascular Aging.

Artery research·2016
Same author

Matrix modulation of compensatory lung regrowth and progenitor cell proliferation in mice.

American journal of physiology. Lung cellular and molecular physiology·2009
Same author

Lysyl oxidase like-1 dysregulation and its contribution to direct inguinal hernia.

European journal of clinical investigation·2009

Related Experiment Video

Updated: Jul 18, 2026

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
07:35

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

Published on: June 9, 2014

Chemotactic activity of elastin-derived peptides.

R M Senior, G L Griffin, R P Mecham

    The Journal of Clinical Investigation
    |October 1, 1980
    PubMed
    Summary

    Elastin peptides attract inflammatory cells, particularly monocytes. This suggests that elastin breakdown, common in emphysema, may signal immune cell recruitment to the lungs.

    Area of Science:

    • Biochemistry
    • Immunology
    • Cell Biology

    Background:

    • Elastin is a key structural protein in connective tissues.
    • Elastin degradation occurs in diseases like emphysema.
    • The role of elastin degradation products in inflammation is not fully understood.

    Purpose of the Study:

    • To investigate the chemotactic activity of elastin-derived peptides.
    • To identify the specific elastin fragments responsible for cell recruitment.
    • To explore the implications for inflammatory diseases.

    Main Methods:

    • Digestion of human and bovine elastin with human neutrophil elastase.
    • Assay of chemotactic activity on monocytes, neutrophils, and alveolar macrophages.
    • Gel filtration chromatography for peptide fractionation.

    More Related Videos

    Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
    11:46

    Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

    Published on: May 19, 2018

    Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method
    06:30

    Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method

    Published on: January 9, 2026

    Related Experiment Videos

    Last Updated: Jul 18, 2026

    Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
    07:35

    Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli

    Published on: June 9, 2014

    Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
    11:46

    Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

    Published on: May 19, 2018

    Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method
    06:30

    Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method

    Published on: January 9, 2026

  • Testing purified desmosine for chemotactic properties.

    • Main Results:

    • Elastin digests exhibited significant chemotactic activity for monocytes.
    • Maximal activity was associated with peptides of 14,000-20,000 mol wt, rich in desmosine cross-links.
    • Purified desmosine also demonstrated monocyte chemotaxis.
    • Serum and anti-elastin antibodies inhibited the chemotactic effect.

    Conclusions:

    • Elastin degradation products, particularly those containing desmosine cross-links, act as chemoattractants for inflammatory cells.
    • Elastin proteolysis may serve as a signal for mononuclear phagocyte recruitment in the lungs.
    • These findings link elastin breakdown in emphysema to inflammatory cell infiltration.