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Rapid grain and cell counting for cell kinetic studies.

P A Bunn, S S Ford, S E Shackney

Journal of the National Cancer Institute

|July 1, 1977

Summary

A bacterial colony counter was adapted to count radioautographic grains and cells in histologic sections. The instrument provided rapid and accurate counts, reducing observer fatigue for both applications.

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Area of Science:

Biotechnology
Histology
Radiography

Background:

Accurate cell and grain counting is crucial in biological research.
Manual counting methods can be time-consuming and prone to fatigue.
Adapting existing laboratory equipment can offer efficient solutions.

Purpose of the Study:

To evaluate a commercial bacterial colony counter for counting radioautographic grains.
To assess the instrument's utility in counting cells within histologic sections.
To compare the efficiency and accuracy of the instrument against manual methods.

Main Methods:

A bacterial colony counter was modified for image analysis.
Radioautographic smears and histologic sections (mouse bone marrow) were analyzed.
Instrument counts were compared with visual counts from multiple observers.

Main Results:

High correlation coefficients (r=0.999 and r=0.998) were observed for radioautographic grain counts compared to visual estimates.
The instrument significantly reduced counting time and observer fatigue for grain analysis.
A good estimation (r=0.968) of bone marrow cell numbers was achieved, with substantially faster data acquisition than semiquantitative methods.

Conclusions:

A bacterial colony counter can be effectively adapted for accurate and efficient counting of radioautographic grains.
The instrument provides a valuable tool for rapid cell enumeration in histologic sections, such as bone marrow cellularity.
This adaptation offers a practical and time-saving alternative to manual counting techniques in biological research.

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