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Related Experiment Videos

Preliminary observations on human trabecular meshwork cells in vitro.

I Grierson, E Robins, R C Howes

    Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie. Albrecht Von Graefe'S Archive for Clinical and Experimental Ophthalmology
    |January 1, 1980
    PubMed
    Summary

    Human trabecular meshwork cells in tissue culture showed metabolic activity but adapted quickly, altering their morphology. This rapid adaptation raises questions about the value of long-term cultures for studying glaucoma.

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    Area of Science:

    • Ophthalmology
    • Cell Biology
    • Tissue Engineering

    Background:

    • The trabecular meshwork (TM) is crucial for regulating intraocular pressure.
    • Understanding TM cell behavior in vitro is vital for glaucoma research.
    • Previous studies have explored TM cell cultures with varying success.

    Purpose of the Study:

    • To observe the behavior and morphology of human trabecular meshwork cells in short-term tissue culture.
    • To assess the metabolic activity and ultrastructural characteristics of cultured TM cells.
    • To evaluate the suitability of long-term TM cell cultures for investigating the glaucomatous outflow system.

    Main Methods:

    • Human donor eyes (up to 5 days post-mortem) were used for explant cultures.
    • Phase-contrast microscopy, time-lapse cinephotomicrography, and electron microscopy were employed.

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  • Autoradiography with tritiated thymidine was used to assess cellular activity.
  • Ultrastructural analysis examined cytoplasmic features like endoplasmic reticulum and mitochondria.

    • Main Results:

    • Satisfactory primary cultures were achieved from approximately 20% of explants.
    • Cultured TM cells exhibited metabolic activity, with abundant organelles.
    • Significant morphological adaptation to the culture environment was observed within short-term cultures.
    • Cultured cells no longer resembled in vivo TM cells.

    Conclusions:

    • Human trabecular meshwork cells are metabolically active in vitro.
    • Rapid morphological adaptation of TM cells in culture limits their resemblance to in vivo cells.
    • The value of long-term TM cell cultures for studying normal and glaucomatous outflow systems is questionable due to adaptation and the presence of non-TM cells.