Frozen resin-cracking, dry-cracking and enzyme-digestion methods in SEM as applied to ocular tissues

Frozen resin-cracking and dry-cracking methods, as well as enzyme digestion by trypsin, hyaluronidase and collagenase, were applied to ocular tissues to study the usefulness of these methods in scanning electron microscopy (SEM). The methods are described and compared, and their advantages and limitations indicated. It is concluded that all the methods presented can give extended information in SEM, but that it is necessary to be familiar with the artifacts produced.