Quantitative TLC determination of propranolol in human plasma

Area of Science:

• Analytical Chemistry
• Pharmacology

Background:

• Accurate quantification of propranolol is crucial for therapeutic drug monitoring.
• Existing methods may have limitations in sensitivity or applicability.

Purpose of the Study:

• To develop and validate a novel spectrodensitometric assay for propranolol determination.
• To assess the assay's sensitivity, linearity, and recovery in biological matrices.

Main Methods:

• Spectrodensitometric analysis of propranolol on silica gel plates.
• Irradiation at 822 nm to measure absorbance.
• Assay validation using spiked plasma samples.

Main Results:

• Detection of propranolol down to 0.10 microgram.
• Linear quantification range established between 0.010 and 0.400 microgram.
• High percent recovery (90.0%–102.0%) from spiked plasma.

Conclusions:

• The developed spectrodensitometric assay is sensitive and reliable for propranolol quantification.
• This method is suitable for determining propranolol levels in patient plasma during therapeutic use.