High-performance liquid chromatographic and TLC determinations of desacetylvinblastine amide (vindesine) and its...

High-performance liquid chromatographic and TLC methods were developed for the determination of desacetylvinblastine amide base (vindesine), a chemically modified Catharanthus alkaloid presently in clinical evaluation. Both methods permit the detection of 6,7-dihydrovindesine and vindesine Nb'-oxide, the principal by-product in the chemical conversion of vinblastine to vindesine. The methods are applicable to both the sulfate salts and the free bases of the chemical substances described.