Kinins in relation to kallikrein activity, kininogen, electrolytes, aldosterone and catecholamines in urine from...

The object of the present study was to test the hypothesis that urinary kinin excretion is an indicator of intrarenal kinin formation and to investigate urinary excretion of kinins in relation to natriuresis, kaliuresis, diuresis and urinary aldosterone and catecholamines in normal individuals on a free salt and water intake. In freshly voided urine collected from 24 normal individuals kinin concentration was directly related to kallikrein activity. Kininogen concentration was very low and neither related to kallikrein activity nor to kinin concentration. The excretion rates of kinins and kininogen were unrelated to the time interval between micturitions. In 24 hour urine collections from 50 normal individuals the excretion of kinins was positively correlated to natriuresis, kaliuresis and diuresis and also to dopamine but not to aldosterone, noradrenaline and adrenaline. Kinin excretion was inversely related to age and was lower in women than in men. On the basis of these results it is concluded that urinary kinin excretion reflects intrarenal kinin formation in normal ambulatory individuals and that urinary kinins are formed mainly in the interstitial and vascular space of the kidney. Furthermore, kinins and dopamine seem to play a physiological role in the renal handling of electrolytes and water.