Non-aerated cultivation of Halobacterium cutirubrum and its effect on cellular squalenes

Halobacterium cutirubrum was successfully cultivated under aerobic and microaerobic conditions. The early stationary phase of growth was obtained at 2.2 days and 45--55 days for aerated and non-aerated cultures, respectively. The dry cell yields were 0.7--1.2 gm/1 in all preparations grown to early stationary growth phase. The cellular ratio of squalene to dihydro- and tetra-hydrosqualene decreased proportionately with decreased aeration rates.