Related Experiment Videos
Antibiotic sensitivity pattern of recent clinical isolates of Streptococcus pneumoniae
Abstract:
Antimicrobial susceptibility of 180 recent isolates of Streptococcus pneumoniae was determined by microdilution technic. There was a high degree of susceptibility to both penicillin G and cefuroxime, except for one strain which required 0.25 microgram/ml. All strains were inhibited by 0.06 microgram/ml of ampicillin, clindamycin and erythromycin. When tested against doxycycline 97.2% of the strains were inhibited by 1.0 microgram/ml. 8 microgram/ml inhibited all strains. Three of the strains were chloramphenicol-resistant with MIC more than 8 microgram/ml. These strains could be shown to inactivate chloramphenicol. All strains but three were susceptible to 20/l microgram/ml of sulfamethoxazole/trimethoprim.
Insights
Most Streptococcus pneumoniae strains show high susceptibility to common antibiotics like penicillin and ampicillin. However, a few strains exhibited resistance to chloramphenicol, indicating potential challenges in treatment.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Streptococcus pneumoniae is a leading cause of bacterial infections worldwide.
- Antimicrobial resistance is a growing public health concern, necessitating regular surveillance of bacterial susceptibility patterns.
- Understanding the susceptibility profiles of S. pneumoniae is crucial for guiding effective treatment strategies.
Purpose of the Study:
- To determine the antimicrobial susceptibility of recent clinical isolates of Streptococcus pneumoniae.
- To evaluate the efficacy of various antibiotics against S. pneumoniae, including penicillin G, cefuroxime, ampicillin, clindamycin, erythromycin, doxycycline, chloramphenicol, and sulfamethoxazole/trimethoprim.
Main Methods:
- Microdilution technique was employed to assess the minimum inhibitory concentrations (MICs) of tested antibiotics.
- 180 recent isolates of Streptococcus pneumoniae were analyzed.
Main Results:
- High susceptibility was observed for penicillin G and cefuroxime, with only one strain showing reduced susceptibility.
- All strains were susceptible to ampicillin, clindamycin, and erythromycin at 0.06 microgram/ml.
- 97.2% of strains were inhibited by 1.0 microgram/ml of doxycycline, and all strains by 8 microgram/ml.
- Three strains demonstrated chloramphenicol resistance (MIC > 8 microgram/ml) due to inactivation of the antibiotic.
- All but three strains were susceptible to sulfamethoxazole/trimethoprim.
Conclusions:
- Recent isolates of Streptococcus pneumoniae remain largely susceptible to penicillin G, cefuroxime, ampicillin, clindamycin, erythromycin, and doxycycline.
- The emergence of chloramphenicol-resistant strains, capable of inactivating the drug, warrants attention.
- Sulfamethoxazole/trimethoprim showed broad susceptibility, with minimal resistance observed.