Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Haemopoietic microenvironments in vitro: ultrastructural aspects.

T D Allen

    Ciba Foundation Symposium
    |January 1, 1981
    PubMed
    Summary

    Long-term bone marrow cultures support various blood cell production. Researchers observed distinct microenvironments influencing granulopoiesis and erythropoiesis, suggesting cell-cell communication in hematopoietic development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A small plant industrial eye program.

    Transactions - American Academy of Ophthalmology and Otolaryngology. American Academy of Ophthalmology and Otolaryngology·2010
    Same author

    The value of the complete routine examination in ophthalmological practice.

    Archivos. Asociacion para Evitar la Ceguera en Mexico·2010
    Same author

    Fundamentals of ophthalmologic office routine.

    Medical record and annals·2010
    Same author

    Refraction with special reference to presbyopia.

    Medical record and annals·2010
    Same author

    Managing the glaucomatous patient.

    Medical record and annals·2010
    Same author

    A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM).

    Nature protocols·2007

    Area of Science:

    • Hematology
    • Cell Biology
    • Microenvironment Research

    Background:

    • Long-term bone marrow cultures (LTBMC) are vital for studying hematopoiesis.
    • Understanding the stromal microenvironment's role is crucial for hematopoietic regulation.

    Purpose of the Study:

    • To ultrastructurally investigate LTBMC from various species.
    • To elucidate the stromal microenvironment's influence on granulopoiesis and erythropoiesis.
    • To explore potential intracellular communication pathways.

    Main Methods:

    • Ultrastructural investigation of LTBMC (human, tree shrew, mouse).
    • Comparative analysis of cultures supporting granulopoiesis versus erythropoiesis.
    • Microscopic examination of stromal elements and hematopoietic cell interactions.

    Main Results:

    • LTBMC support diverse hematopoietic lineages, including granulocytes and erythroid series.
    • Stromal microenvironments differ based on differentiation: granulocytic cultures show fat cell association, while erythropoietic cultures have reduced fat cells and increased monocytic association.
    • In vitro transmural passage of mature granulocytes and monocytes through endothelial layers was observed.
    • Erythropoiesis involves close association with monocytic cells, forming erythroblastic islets.

    Conclusions:

    • The stromal microenvironment in LTBMC is inductive and specific to hematopoietic differentiation.
    • Distinct stromal features correlate with granulopoiesis and erythropoiesis.
    • Evidence suggests intracellular communication between hematopoietic cells and stromal components.

    Related Experiment Videos