Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Murine complement factor B (BF): sexual dimorphism and H-2-linked polymorphism.

M H Roos, P Démant

    Immunogenetics
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    TheH-2L locus and the system of H-2 specificities.

    Immunogenetics·2011
    Same author

    Preglomerular and postglomerular basal diameter changes and reactivity to angiotensin II in obese rats.

    Diabetes, obesity & metabolism·2007
    Same author

    Mouse genetic model for antigen-induced airway manifestations of asthma.

    Genes and immunity·2006
    Same author

    Hepatocellular carcinoma in Txnip-deficient mice.

    Oncogene·2006
    Same author

    Genetics of susceptibility to leishmaniasis in mice: four novel loci and functional heterogeneity of gene effects.

    Genes and immunity·2006
    Same author

    Genetic control of Bordetella pertussis infection: identification of susceptibility loci using recombinant congenic strains of mice.

    Infection and immunity·2005

    Genetic analysis revealed polymorphism in murine BF (Factor B) proteins, linked to the H-2 complex. Sexual dimorphism was observed in BF band patterns between male and female mice.

    Area of Science:

    • * Immunology
    • * Genetics
    • * Molecular Biology

    Background:

    • * The murine Major Histocompatibility Complex (MHC), known as the H-2 complex in mice, plays a crucial role in immune responses.
    • * Factor B (BF) is a component of the alternative pathway of complement activation and its genetic variations can impact immune function.
    • * Understanding the genetic organization and regulation of the H-2 complex is vital for immunological research.

    Purpose of the Study:

    • * To investigate the genetic polymorphism of murine Factor B (BF).
    • * To determine the genetic linkage of BF variants to the H-2 complex.
    • * To explore potential sexual dimorphism in BF expression patterns.

    Main Methods:

    • * Agarose gel electrophoresis of EDTA-plasma from various mouse strains.

    Related Experiment Videos

  • * Western blotting of separated proteins onto cellulose nitrate sheets.
  • * Detection of BF using specific anti-BF serum, anti-IgG serum, and 125I-labeled Protein A, followed by autoradiography.

    • Main Results:

    • * Four or five distinct BF bands were identified in plasma of male mice.
    • * A more anodal BF variant was observed in the WLL/BrA (H-2bs) strain compared to other tested strains.
    • * BF variants consistently cosegregated with H-2 haplotypes in backcross and F2 generations, establishing linkage.
    • * A sexual dimorphism was detected, with females exhibiting three main BF bands versus four or five in males, though protein levels were comparable.

    Conclusions:

    • * The study establishes a genetic linkage between murine BF polymorphism and the H-2 complex.
    • * A sexual dimorphism in BF electrophoretic patterns between male and female mice was identified.
    • * These findings contribute to understanding MHC interspecies homology and offer avenues for studying H-2 complex genetic organization and hormonal regulation.