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Subtyping of properdin factor B (Bf) by isoelectrofocusing
Human Heredity
|January 1, 1982
Summary
Properdin factor B (Bf) protein subtypes were identified in human plasma. New alleles, BfFa and BfFb, were discovered and characterized, adding to the known BfS, BfF, BfF1, and BfS1 variants.
Area of Science:
- Human genetics
- Biochemistry
- Immunology
Background:
- Properdin factor B (Bf) is a polymorphic protein found in human plasma.
- Previous studies identified common (BfS, BfF) and rare (BfF1, BfS1) alleles in Caucasian populations.
Purpose of the Study:
- To investigate further polymorphism within the BfF allele.
- To characterize newly identified Bf alleles and determine their inheritance patterns.
Main Methods:
- Isoelectrofocusing was employed to subtype the BfF allele.
- Family studies were conducted to analyze the inheritance of the identified alleles.
Main Results:
- The BfF allele was successfully subtyped into two distinct alleles: BfFa and BfFb.
- Family studies confirmed that BfFa and BfFb are inherited in a co-dominant pattern.
- Gene frequencies for Caucasian populations were determined: BfS (0.808), BfF1 (0.019), BfS1 (0.005), BfFa (0.038), and BfFb (0.128).
Conclusions:
- The human Properdin factor B (Bf) system exhibits greater polymorphism than previously recognized.
- The identification and characterization of BfFa and BfFb alleles contribute to a more comprehensive understanding of Bf genetic variation.