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[Effect of pinocembrin on the course of experimental candida infections in mice]
Abstract:
Pinocembrin (5,7-dihydroxy-flavanon)--a component of the bee product propolis--was tested for its in vivo activity against Candida albicans in mice. It was shown that the intravenous infection of AB-Jena mice with 2.5 X 10(5) Candida albicans cells was a very suitable model. Despite of treatment with pinocembrin at daily doses of 100 mg/kg body weight the animals as well as the controls died between the 6th and 24th day after beginning. On the other hand the animals treated with 5 mg/kg amphotericin B survived the test-period of 30 days. The question of effectiveness of pinocembrin in vivo should be cleared up in further pharmacokinetic investigations.
Insights
Pinocembrin, a propolis component, showed no significant in vivo antifungal activity against Candida albicans in mice. Further pharmacokinetic studies are needed to clarify its effectiveness.
Area of Science:
- Natural Products Chemistry
- Mycology
- Pharmacology
Context:
- Candida albicans is a common fungal pathogen causing opportunistic infections.
- Propolis, a bee product, contains various bioactive compounds, including pinocembrin.
- Evaluating natural compounds for antifungal activity is crucial for developing new treatments.
Purpose:
- To investigate the in vivo antifungal efficacy of pinocembrin against Candida albicans.
- To establish a suitable mouse model for testing antifungal agents.
Summary:
- An intravenous Candida albicans infection model in AB-Jena mice was utilized.
- Mice treated with pinocembrin (100 mg/kg daily) did not survive the observation period.
- Amphotericin B (5 mg/kg) served as a positive control, with treated mice surviving 30 days.
Impact:
- Pinocembrin demonstrated limited in vivo antifungal activity in this study.
- Further pharmacokinetic investigations are required to understand pinocembrin's behavior in vivo.
- This research highlights the need for rigorous testing of natural compounds as therapeutic agents.