Related Experiment Video
Updated: Aug 7, 2026
09:47
Isolation, Processing and Analysis of Murine Gingival Cells
Published on: July 2, 2013
Gingival matrix collagen in chronic periodontitis
Journal of Dental Research
|January 1, 1980
Summary
Chronic periodontitis alters human gingival collagen, reducing Type III collagen and changing non-collagenous proteins. These findings highlight key molecular differences in diseased gum tissue.
Area of Science:
- Oral biology
- Biochemistry
- Periodontology
Background:
- Chronic periodontitis is a prevalent inflammatory disease affecting gum tissues.
- Collagen is a major structural protein in connective tissues, including gingiva.
- Alterations in gingival collagen composition may contribute to disease pathogenesis.
Purpose of the Study:
- To characterize the collagen and non-collagenous protein components of human gingival tissue from patients with chronic periodontitis.
- To compare the composition of diseased gingival tissue with normal gingival tissue.
Main Methods:
- Isolation and characterization of cyanogen bromide peptides from human gingival collagen.
- Analysis of collagen types (Type I and Type III) present in the tissue.
- Characterization of the non-collagenous protein fraction.
- Amino acid analysis of both normal and diseased gingival tissues.
Main Results:
- Human gingival tissue in chronic periodontitis contains both Type I and Type III collagens.
- A reduced amount of Type III collagen was observed in diseased tissue compared to normal tissue.
- A non-collagenous protein fraction, comprising about 20% of the insoluble matrix, was rich in acidic, hydrophobic, and hydroxy-containing amino acids.
- Significant qualitative and quantitative differences in amino acid composition were found between normal and diseased gingival tissues.
Conclusions:
- Chronic periodontitis is associated with changes in the composition of human gingival collagen, specifically a decrease in Type III collagen.
- The non-collagenous matrix of diseased gingival tissue exhibits distinct amino acid profiles.
- These molecular alterations in gingival tissue may play a role in the progression of chronic periodontitis.
Related Concept Videos
01:42The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
01:29The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
01:21Matrix Proteoglycans and Glycoproteins
Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
01:23Role of Matrix Metalloproteases in Degradation of ECM
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
A...
01:28Phases of Wound Repair
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
01:26Extracellular Matrix
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...