Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hemoglobin switching: a cellular model.

B P Alter, S C Goff

    Science (New York, N.Y.)
    |February 8, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Hemoglobin synthesis regulation is influenced by cell populations. Researchers found that specific mouse erythroleukemia cell clones, when induced, demonstrated varying levels of beta minor and beta major hemoglobin production, indicating cellular-level control.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    VACTERL-H Association and Fanconi Anemia.

    Molecular syndromology·2013
    Same author

    Disease-specific hematopoietic cell transplantation: nonmyeloablative conditioning regimen for dyskeratosis congenita.

    Bone marrow transplantation·2010
    Same author

    Oral and dental phenotype of dyskeratosis congenita.

    Oral diseases·2008
    Same author

    Griscelli syndrome: rare neonatal syndrome of recurrent hemophagocytosis.

    Journal of pediatric hematology/oncology·2002
    Same author

    Modern review of congenital hypoplastic anemia.

    Journal of pediatric hematology/oncology·2001
    Same author

    Comparative genome analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster.

    Human molecular genetics·2001
    Same journal

    Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

    Science (New York, N.Y.)·2026
    Same journal

    Local signals, systemic decline.

    Science (New York, N.Y.)·2026
    Same journal

    The mechanics of liver regeneration.

    Science (New York, N.Y.)·2026
    Same journal

    Computing in a memory with physics.

    Science (New York, N.Y.)·2026
    Same journal

    Retraction.

    Science (New York, N.Y.)·2026
    Same journal

    Making time.

    Science (New York, N.Y.)·2026
    See all related articles

    Area of Science:

    • Molecular Biology
    • Hematology
    • Cell Biology

    Background:

    • Hemoglobin synthesis is crucial for oxygen transport.
    • Regulation of hemoglobin production involves complex cellular mechanisms.
    • Erythroid differentiation is a key process in red blood cell development.

    Purpose of the Study:

    • To investigate the role of cellular populations in regulating hemoglobin synthesis.
    • To determine the relative induction of beta minor and beta major hemoglobin in cloned mouse erythroleukemia cells.

    Main Methods:

    • Cloning of mouse erythroleukemia cells (line 745).
    • Induction of cloned cells with dimethyl sulfoxide (DMSO).
    • Quantification of beta minor and beta major hemoglobin synthesis post-induction.

    Related Experiment Videos

    Main Results:

    • Initial clones showed variable beta minor synthesis (23-61%) after DMSO induction.
    • Subcloning a high-producing clone resulted in cell lines synthesizing over 90% beta minor.
    • Demonstrated significant variation in hemoglobin synthesis among different cell clones.

    Conclusions:

    • Hemoglobin synthesis regulation can occur at the cellular level.
    • Cellular heterogeneity plays a role in the differential expression of globin genes.
    • These findings provide insights into the molecular mechanisms controlling erythropoiesis.