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[Plasmid resistance of the enterobacteria isolated in intestinal disorders in children]
Abstract:
Resistance to antibiotics and sulfanilamides was determined in enterobacteria (E. coli, Pr. mirabilis, Sh. sonnei, Sh. flexneri) isolated from children with intestinal disorders. Elimination of the resistance determinants in polyresistant strains, conjugation of R plasmids (75 per cent in Pr. mirabilis, 70 per cent in E. coli, 78 per cent in Sh. sonnei, 69 per cent in Sh. flexneri), mobilization of nonconjugative plasmids on triple crossing and capacity of R plasmids for repression of the F-factor functions were studied. The data of the study are evident of the plasmid background in enterobacteria isolated from children with intestinal disorders and confirm the plasmid nature of the resistance in the predominating number of the strains. It is suggested that Proteus plays a significant role in the prevalence of plasmid resistance among enterobacteria.
Insights
Antibiotic resistance in enterobacteria from children with intestinal disorders is often plasmid-mediated. Proteus species may significantly contribute to the spread of this plasmid resistance among enterobacteria.
Area of Science:
- Microbiology
- Genetics
- Infectious Diseases
Context:
- Enterobacteria, including E. coli, Pr. mirabilis, Sh. sonnei, and Sh. flexneri, were isolated from children experiencing intestinal disorders.
- Antibiotic and sulfanilamide resistance patterns were analyzed in these clinical isolates.
Purpose:
- To investigate the prevalence and nature of antibiotic and sulfanilamide resistance in enterobacteria from pediatric intestinal disorders.
- To elucidate the role of plasmids in conferring resistance and to study plasmid transfer mechanisms (conjugation, mobilization).
- To assess the potential contribution of Proteus species to the dissemination of resistance.
Summary:
- Resistance to antibiotics and sulfanilamides was evaluated in enterobacteria from children with intestinal disorders.
- Plasmid-mediated resistance was confirmed in a majority of polyresistant strains, with high rates of R plasmid conjugation observed across different species (Pr. mirabilis, E. coli, Sh. sonnei, Sh. flexneri).
- Mobilization of nonconjugative plasmids and repression of F-factor functions by R plasmids were also studied, indicating a significant plasmid background.
Impact:
- The study highlights the substantial role of plasmids in antibiotic resistance among enterobacteria in pediatric intestinal infections.
- Findings suggest that Proteus species may be key players in the dissemination of plasmid-borne resistance within enterobacterial populations.
- This research underscores the importance of understanding plasmid dynamics for combating antimicrobial resistance in clinical settings.