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Human gene mapping using an X/autosome translocation.

E Solomon, M Bobrow, P N Goodfellow

    Somatic Cell Genetics
    |March 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

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    Human fibroblasts with an X/15 chromosome translocation were used to map genes. This study successfully assigned several markers, including N-acetyl hexosaminidase A (HEXA), to chromosome 15.

    Area of Science:

    • Genetics
    • Molecular Biology
    • Cell Biology

    Background:

    • Human fibroblasts with a specific X chromosome and chromosome 15 translocation were utilized.
    • A mouse cell line resistant to 6-thioguanine (IR) was employed for cell fusion experiments.

    Purpose of the Study:

    • To map genes on the human X chromosome and chromosome 15 using somatic cell hybrids.
    • To confirm the location of X-linked and non-X-linked markers through segregation analysis.

    Main Methods:

    • Somatic cell hybridization of human fibroblasts and mouse IR cells.
    • Selection of hybrid cells in HAT medium and counterselection in 6-thioguanine.
    • Analysis of marker segregation (G6PD, PGK, HPRT, PKM2, MPI, HEXA, beta2-m) using electrophoretic and immunochemical techniques.

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    Main Results:

    • Hybrid cells retaining the X/15 translocation chromosome were successfully generated and selected.
    • X-linked markers (G6PD, PGK, HPRT) and non-X-linked markers (PKM2, MPI, HEXA, beta2-m) segregated concordantly with the X/15 translocation.
    • The non-X-linked markers were assigned to human chromosome 15.

    Conclusions:

    • The study successfully assigned several genes to human chromosome 15 based on segregation analysis in somatic cell hybrids.
    • The X/15 translocation chromosome served as a useful tool for gene mapping.
    • Further characterization of N-acetyl hexosaminidases A and B was performed in these hybrids.