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Related Experiment Videos

Cell density determines epithelial migration in culture.

P Rosen, D S Misfeldt

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1980
    PubMed
    Summary

    Dog kidney epithelial cells (MDCK) show density-dependent migration. Cell sheets accelerate into wounds only after reaching a specific threshold density, indicating density regulates cell sheet movement.

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    Area of Science:

    • Cell Biology
    • Epithelial Cell Biology
    • Wound Healing Models

    Background:

    • Madin-Darby Canine Kidney (MDCK) cells exhibit density-correlated growth inhibition.
    • Confluent MDCK monolayers display cell sheet migration into wounded areas.

    Purpose of the Study:

    • To investigate the relationship between cell density and migration dynamics in MDCK cell sheets.
    • To determine the threshold cell density required for initiating cell sheet migration and acceleration.

    Main Methods:

    • Wounding confluent and sub-confluent MDCK monolayers at various cell densities.
    • Precisely measuring cell migration rates and acceleration over time.
    • Analyzing the role of cell density in regulating the initiation and maintenance of cell sheet movement.

    Main Results:

    • MDCK cell sheets migrated into wounded areas with a uniform acceleration of 0.24 micrometer/hr².
    • Migration initiation required reaching a threshold density of 2.0 X 10⁵ cells/cm², irrespective of initial wounding density.
    • Cell migration balanced cell division to maintain this threshold density during movement.

    Conclusions:

    • Cell sheet migration is initiated at a specific, density-dependent threshold.
    • The observed constant acceleration suggests a general characteristic of vectorial epithelial cell sheet movement.
    • Cell density plays a crucial role in regulating the initiation and sustained movement of epithelial cell sheets.

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