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Sertoli cells contain a mitogenic polypeptide.

L A Feig, A R Bellvé, N H Erickson

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1980
    PubMed
    Summary
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    Mouse testes contain a potent growth factor that stimulates cell division in cultured cells. This testicular mitogen, primarily found in prepuberal Sertoli cells, is a heat-sensitive polypeptide.

    Area of Science:

    • Reproductive Biology
    • Cell Biology
    • Biochemistry

    Background:

    • The mammalian testis contains factors influencing cell proliferation.
    • Understanding testicular cell growth is crucial for reproductive health research.

    Purpose of the Study:

    • To identify and characterize a mitogenic factor present in the mouse seminiferous epithelium.
    • To investigate the source and properties of this testicular growth factor.

    Main Methods:

    • Culturing quiescent BALB/c 3T3 cells.
    • Assaying mitogenic activity using [methyl-3H]thymidine incorporation.
    • Utilizing gel-filtration chromatography and protease sensitivity assays.

    Main Results:

    • Seminiferous tubule homogenates induce significant DNA synthesis and cell division in 3T3 cells.

    Related Experiment Videos

  • Mitogenic activity is higher in newborn mouse testes and prepuberal Sertoli cells.
  • The factor is a heat-labile polypeptide with an approximate molecular weight of 15,500.

    • Conclusions:

    • A potent mitogenic polypeptide growth factor exists in the mammalian testis.
    • Prepuberal Sertoli cells are a rich source of this testicular growth factor.
    • This finding contributes to understanding testicular cell regulation and growth factor biology.