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Evidence for unequal crossing over within the mouse T/t complex.

L M Silver, M White, K Artzt

    Proceedings of the National Academy of Sciences of the United States of America
    |October 1, 1980
    PubMed
    Summary

    Researchers studied the Tcp-1 gene in mice, finding that rare recombination events can link different Tcp-1 alleles (Tcp-1a and Tcp-1b) on the same chromosome. This suggests significant DNA sequence differences within the T/t complex.

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    Area of Science:

    • Genetics
    • Molecular Biology
    • Mammalian Genetics

    Background:

    • The Tcp-1 gene, located in the mouse T/t complex on chromosome 17, encodes the p63/6.9 protein.
    • Two structural alleles, Tcp-1a and Tcp-1b, specify alternate forms of this protein.
    • Tcp-1b is found on wild-type chromosome 17, while Tcp-1a is associated with t haplotype chromosomes.

    Purpose of the Study:

    • To analyze rare recombinant chromosomes in mice heterozygous for T/t haplotypes.
    • To investigate the association of Tcp-1 alleles (Tcp-1a and Tcp-1b) in these recombinant events.
    • To explore the genetic basis for recombination suppression and unequal crossing over within the T/t complex.

    Main Methods:

    • Analysis of 15 rare recombinant chromosomes derived from mice heterozygous for complete t haplotypes.

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  • Genetic analysis of Tcp-1 alleles (Tcp-1a and Tcp-1b) in recombinant DNA molecules.
  • Comparative analysis of DNA sequence arrangements between wild-type and t-carrying chromosome 17.

    • Main Results:

    • Four independent rare recombination events resulted in the association of Tcp-1b and Tcp-1a alleles in a cis position on a single DNA molecule.
    • These findings support a hypothesis of significant nonhomology in DNA sequence arrangement between wild-type and t-carrying chromosome 17.
    • The observed nonhomology may explain both the suppression of normal recombination and the occurrence of unequal crossing over within the T/t complex.

    Conclusions:

    • Rare crossing over events within the T/t complex can lead to the cis-association of distinct Tcp-1 alleles.
    • Significant DNA sequence nonhomology between wild-type and t-haplotype chromosomes underlies recombination patterns.
    • This genetic variation provides insights into the structural organization and evolutionary dynamics of the mouse T/t complex.