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Functional integrity of cryopreserved human monocytes.

R S Weiner, S J Normann

    Journal of the National Cancer Institute
    |February 1, 1981
    PubMed
    Summary

    Cryopreservation of human monocytes using 7.5-10% dimethyl sulfoxide (DMSO) effectively preserves their function. Cryopreserved monocytes maintain significant chemotactic and metabolic activity, crucial for immunological studies.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Cryobiology

    Background:

    • Human peripheral blood monocytes are critical immune cells.
    • Cryopreservation is essential for preserving cells for research.
    • Dimethyl sulfoxide (DMSO) is a common cryoprotective agent.

    Purpose of the Study:

    • To determine optimal dimethyl sulfoxide (DMSO) concentrations for cryopreserving human monocytes.
    • To evaluate the functional recovery of cryopreserved human monocytes.

    Main Methods:

    • Human peripheral blood monocytes were collected via semicontinuous flow centrifugation.
    • Cells were cryopreserved using varying concentrations of DMSO.
    • Chemotactic responsiveness, fructose-6-phosphate shunt activity, and cytotoxic activity were assessed.

    Main Results:

    • Optimal cryoprotection was achieved with 7.5-10% DMSO.
    • Cryopreserved monocytes retained 80% of their chemotactic responsiveness and fructose-6-phosphate shunt activity.
    • Both fresh and cryopreserved monocytes exhibited comparable, low levels of cytotoxic activity.

    Conclusions:

    • 7.5-10% DMSO provides effective cryopreservation for human monocytes.
    • Cryopreserved monocytes largely retain key immune functions essential for research.
    • Cryopreservation is a viable method for storing monocytes for immunological assays.

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