Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Covalent binding and hemolytic activity of complement proteins

S K Law, N A Lichtenberg, R P Levine

    Proceedings of the National Academy of Sciences of the United States of America
    |December 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Are face masks useful for limiting the spread of COVID-19?

    Hong Kong medical journal = Xianggang yi xue za zhi·2020
    Same author

    Is reinfection possible after recovery from COVID-19?

    Hong Kong medical journal = Xianggang yi xue za zhi·2020
    Same author

    Cigarette smoking and glaucoma in the United States population.

    Eye (London, England)·2018
    Same author

    Transcription and translation for carotenoid synthesis in Chlamydomonas reinhardtii.

    Planta·2014
    Same author

    Differences in the compliance with hospital infection control practices during the 2009 influenza H1N1 pandemic in three countries.

    The Journal of hospital infection·2012
    Same author

    Comparison of long-term surgical success of Ahmed Valve implant versus trabeculectomy in open-angle glaucoma.

    The British journal of ophthalmology·2009

    Hydroxylamine inactivates the third component of complement (C3), directly correlating hemolytic and binding activities. This supports a model where C3b uses a reactive thioester to bind surfaces.

    Area of Science:

    • Biochemistry
    • Immunology
    • Complement System

    Background:

    • The third component of complement (C3) plays a crucial role in the immune response.
    • Understanding the mechanism of C3 activation and its interaction with surfaces is vital for immunology.

    Purpose of the Study:

    • To investigate the inactivation of C3 by hydroxylamine and methylamine.
    • To elucidate the mechanism of covalent binding of C3b to receptive surfaces.

    Main Methods:

    • Chemical inactivation of C3 using hydroxylamine and [14C]methylamine.
    • Assaying C3 hemolytic activity and covalent binding activity.
    • Quantifying the labeling of C3 in the C3d domain.

    Main Results:

    • Hydroxylamine directly inactivates C3, with identical kinetics for hemolytic and covalent binding activities.

    Related Experiment Videos

  • C3 inactivation correlates quantitatively with [14C]methylamine labeling in the C3d domain.
  • The proposed model of internal thioester reactivity in C3b binding also applies to C4 but not C5.

    • Conclusions:

    • Covalent, surface-bound C3b is hemolytically active.
    • An internal thioester in C3 becomes reactive upon activation to C3b, mediating covalent surface binding.
    • This thioester mechanism is conserved in C4 but absent in C5.