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[Specific karyotype changes in cells resistant to colchicine]

B P Kopnin

    Genetika
    |January 1, 1981
    PubMed
    Summary

    New Djungarian hamster cell sublines exhibit high colchicine resistance due to an unstable marker chromosome. This resistance is linked to gene amplification, as evidenced by homogeneously colored regions (HCR) and associated chromosomal abnormalities.

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    Area of Science:

    • Cell Biology
    • Genetics
    • Molecular Biology

    Context:

    • Djungarian hamster DM-15 cells were used to develop drug-resistant sublines.
    • Colchicine resistance is a significant factor in cancer chemotherapy and drug development.

    Purpose:

    • To characterize new colchicine-resistant Djungarian hamster cell sublines.
    • To investigate the genetic and chromosomal basis of colchicine resistance.

    Summary:

    • Two new sublines, DM-15CHR-1/1 and DM-15CHR-1/5, display 170-190 fold resistance to colchicine compared to parental DM-15 cells.
    • This drug resistance is unstable, with a loss rate of approximately 2.10(-2) per cell per generation under nonselective conditions.
    • Both sublines share a common karyotypic alteration: an additional marker chromosome, a derivative of chromosome 4, containing a homogeneously colored region (HCR) in its long arm. The HCR size varies, averaging 4-5% of total chromosome length.
    • Additional chromosomal abnormalities, including circular chromosomes and double minutes composed of HCR material, were observed in some cells.
    • These findings strongly suggest that colchicine resistance in these cells is associated with gene amplification.

    Impact:

    • Provides a model system for studying drug resistance mechanisms.
    • Contributes to understanding gene amplification and its role in cellular adaptation.
    • Informs strategies for overcoming drug resistance in therapeutic applications.

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