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The polyribosomal mRNA--protein complex is a dynamic structure

J R Greenberg

    Proceedings of the National Academy of Sciences of the United States of America
    |May 1, 1981
    PubMed
    Summary
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    Messenger RNA (mRNA)-associated proteins are synthesized and bind to mRNA, even when mRNA synthesis is blocked. These proteins likely play a role in translation and are added to mRNA in the cytoplasm.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Messenger RNA (mRNA) metabolism is crucial for gene expression.
    • Proteins associated with mRNA play roles in its processing, transport, and translation.
    • Understanding the dynamics of mRNA-protein interactions is key to deciphering gene regulation.

    Purpose of the Study:

    • To investigate the metabolism and dynamics of mRNA-protein complexes in mouse L cells.
    • To identify proteins associated with mRNA and their synthesis under varying conditions.
    • To explore the potential role of these proteins in mRNA translation.

    Main Methods:

    • Utilized ultraviolet light-induced crosslinking to study protein-RNA interactions.
    • Investigated protein association with mRNA during active and inhibited mRNA synthesis.

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  • Analyzed the labeling kinetics of mRNA-associated proteins.

    • Main Results:

    • Identified a set of at least seven proteins that associate with mRNA.
    • These proteins are synthesized and bind to mRNA even when mRNA synthesis is inhibited by actinomycin D.
    • A specific protein (Mr 78,000) was found to crosslink to poly(A) tails of mRNA.
    • Labeling kinetics suggest cytoplasmic addition and a half-life exceeding 2 hours for these proteins.

    Conclusions:

    • mRNA-associated proteins likely exchange with a cytoplasmic pool of free proteins.
    • These proteins are suggested to play a role in the translation process.
    • The findings provide insights into the dynamic nature and functional significance of mRNA-protein complexes.