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[Antibiograms of Leptospira on solid media in Petri dishes]
Abstract:
The sensitivity of fowl leptospirae to antibiotics was tested by diffusiometry on solid media in Petri dishes. The method includes two stages: 1) visible leptospirae cultures are obtained by central seeding and incubation during 3--5 days at 28 or 37 degrees C; 2) antibiotic tablets are disposed at 2 cm from the visible edge of the multiplication zone of the leptospirae. The edge is marked with a pencil at the moment in which the antibiotics are applied. Incubation is continued at the same temperatures. The results are read after five days taking as reference the position of the marginal multiplication line. The sensitivity of leptospirae to antibiotics is expressed by arrested multiplication and resistance by advance of the marginal multiplication line up to or even beyond the antibiotic tablets. Fowl leptospirae are sensitive to penicillin, tetracyclin, chloramphenicol, streptomycin, novobiocin, polymixin, pristinamycin, neomycin, erythromycin, and resistant to mycostatin, septrin, rifampicin, optoquine, sulphatiazol.
Insights
Fowl leptospirae show sensitivity to common antibiotics like penicillin and tetracycline. However, they are resistant to certain drugs including mycostatin and rifampicin, indicating varied antibiotic responses.
Area of Science:
- Veterinary Microbiology
- Bacteriology
Context:
- Leptospirosis is a significant zoonotic disease affecting poultry.
- Understanding antibiotic susceptibility in fowl-associated Leptospira is crucial for disease management.
Purpose:
- To evaluate the in vitro antibiotic sensitivity of fowl-derived Leptospira isolates.
- To determine resistance patterns against a panel of commonly used antibiotics.
Summary:
- A diffusiometry method on solid media was employed to test antibiotic sensitivity.
- Fowl Leptospira cultures were incubated, and antibiotic tablets were applied to assess growth inhibition zones.
- Results indicated sensitivity to penicillin, tetracycline, chloramphenicol, streptomycin, novobiocin, polymixin, pristinamycin, neomycin, and erythromycin.
- Resistance was observed against mycostatin, septrin, rifampicin, optoquine, and sulphatiazol.
Impact:
- Provides essential data for selecting effective antibiotic treatments for leptospirosis in poultry.
- Highlights potential therapeutic limitations for specific antibiotic classes in fowl leptospira infections.