Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Subcellular localization and secretion of factor V from human platelets

C M Chesney, D Pifer, R W Colman

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1981
    PubMed
    Summary

    Platelets store factor V (FV) in alpha granules. Physiological stimuli trigger FV release, accelerating blood coagulation by providing a receptor for factor Xa.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Residues F16-G33 and A784-N823 within platelet thrombospondin-1 play a major role in binding human neutrophils: evaluation by two novel binding assays.

    The Journal of laboratory and clinical medicine·2000
    Same author

    Effect of niacin, warfarin, and antioxidant therapy on coagulation parameters in patients with peripheral arterial disease in the Arterial Disease Multiple Intervention Trial (ADMIT).

    American heart journal·2000
    Same author

    Re: marker of inflammatory bowel disease.

    The American journal of gastroenterology·2000
    Same author

    Different mechanisms define the antiadhesive function of high molecular weight kininogen in integrin- and urokinase receptor-dependent interactions.

    Blood·2000
    Same author

    Cleaved high molecular weight kininogen binds directly to the integrin CD11b/CD18 (Mac-1) and blocks adhesion to fibrinogen and ICAM-1.

    Blood·2000
    Same author

    Inactivation of platelet PDE2 by an affinity label: 8-[(4-bromo-2, 3-dioxobutyl)thio]cAMP.

    Thrombosis research·2000

    Area of Science:

    • Hematology
    • Biochemistry
    • Cell Biology

    Background:

    • Factor V (FV) is a plasma protein essential for thrombin generation.
    • Platelets contain significant concentrations of FV, but its localization and release mechanisms were unclear.

    Purpose of the Study:

    • To investigate the subcellular localization of platelet factor V.
    • To determine the mechanisms and regulation of factor V secretion from platelets.

    Main Methods:

    • Subcellular fractionation of human platelets.
    • Stimulation of platelets with ADP, epinephrine, and collagen.
    • Assessment of factor V release using biochemical assays.
    • Inhibition studies using aspirin, antimycin A, and 2-deoxyglucose.

    Related Experiment Videos

    Main Results:

    • Factor V is localized within platelet alpha granules.
    • ADP and epinephrine induce partial release of factor V, inhibited by aspirin.
    • Collagen induces a greater release of factor V, partially inhibited by aspirin.
    • Secretion of factor V is dependent on cellular metabolic energy.

    Conclusions:

    • Platelet factor V is stored in alpha granules and secreted upon stimulation by physiological agents.
    • Released factor V can accelerate blood coagulation by acting as a receptor for factor Xa.
    • Platelet factor V secretion is an active, energy-dependent process.