Transformation of bile acids by Eubacterium lentum

A group of fecal isolates identified as Eubacterium lentum elaborated 3 alpha-, 7 alpha-, and 12 alpha-dehydrogenases and also an epimerizing enzyme(s) for the 3 alpha-hydroxy group. The activities of the enzymes, however, were variably manifested according to the kind of bile acid substrate and the oxygen tension under which the reaction occurred.