A radioimmunoassay for 6-ketoprostaglandin E1

A radioimmunoassay for 6-ketoprostaglandin E1 has been developed. 6-keto-prostaglandin E1 antibodies were produced in rabbits by repeated immunization with 6-ketoprostaglandin E1 coupled to bovine serum albumin. [125]-labeled hapten with high specific radioactivity was prepared by radioiodination of 6-ketoprostaglandin in E1-tyrosine methyl ester conjugate followed by purification with thin layer chromatography. The antibodies showed good specificity toward 6-ketoprostaglandin E1 and crossreacted only significantly with prostaglandin E1. The sensitivity of the assay was 10 pg per assay tube. Application of the radioimmunoassay was demonstrated by the detection of immunoreactive 6-ketoprostaglandin E1 from 6-ketoprostaglandin F1 alpha catalyzed by swine renal NADP+-linked 9-hydroxyprostaglandin dehydrogenase.