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Phase transitions in phosphatidylcholine multibilayers

P W Westerman, M J Vaz, L M Strenk

    Proceedings of the National Academy of Sciences of the United States of America
    |May 1, 1982
    PubMed
    Summary

    Deuterium NMR reveals molecular motion in lipid bilayers. Cholesterol influences lipid dynamics, showing hindered rotation and flipping above the pretransition temperature.

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    Journal of lipid research·2000

    Area of Science:

    • Biophysics
    • NMR Spectroscopy
    • Lipid Bilayer Dynamics

    Background:

    • Lipid bilayers are fundamental to cell membranes.
    • Understanding lipid molecular motion is crucial for membrane function.
    • Cholesterol's role in modulating lipid dynamics is of significant interest.

    Purpose of the Study:

    • To investigate the molecular dynamics of a specific phospholipid (1-myristoyl-2-[14,14,14-(2H3)]myristoyl-sn-glycero-3-phosphocholine) in a multilamellar dispersion.
    • To elucidate the effect of cholesterol on lipid molecular motion using Deuterium NMR (2H NMR).
    • To correlate NMR findings with known structural phases of lipid bilayers.

    Main Methods:

    • Recording 2H NMR spectra of a deuterated phospholipid with 1 mol% cholesterol.
    • Analyzing spectral data to obtain motionally averaged quadrupole coupling constants and asymmetry parameters.
    • Conducting experiments across a temperature range from -15°C to 36°C.

    Main Results:

    • Below -4°C, molecular rotation about the long axis is slow on the NMR timescale.
    • Above the pretransition, fast, hindered rotational motion (180° flip) about the molecular long axis is observed.
    • Between -4°C and the pretransition, spectra suggest either two populations of molecules or a single population undergoing flipping motion.
    • In the absence of cholesterol, more complex spectral patterns correlate with observed ripple structures.

    Conclusions:

    • Cholesterol significantly influences lipid molecular motion, promoting hindered rotation and flipping above the pretransition.
    • The observed spectral changes are consistent with distinct phases and structural transitions in lipid bilayers.
    • NMR data provide insights into the molecular basis of lipid-cholesterol interactions and phase behavior.

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