Changes in thromboxane B2 and 6-keto-PGF1 alpha after direct electrical stimulation of the denervated rat...

Radio-immunoassay was used to measure the amounts of Thromboxane B2 (TXB2) and 6-keto-PGF1 alpha produced in the gastrocnemius muscles of rats after denervation and direct electrical stimulation (2-4 mA intensity and 4 msec duration pulses given at 10 Hz continuously, 8 hours each day for 14 days). Compared to the normal control side, the normal-stimulated, the denervated control and the denervated-stimulated muscles, all indicated increases in the content (ng/g muscle) of TXB2 (120-155%, P less than 0.01) and 6-keto-PGF1 alpha (90-10 7%). This study suggests that electrical stimulation as well as denervation may lead to an elevation of these prostanoids.