Related Experiment Videos
H-2 expression on a teratocarcinoma-derived cell line, TerC
Journal of the National Cancer Institute
|December 1, 1982
Summary
The TerC cell line expresses low levels of major histocompatibility locus H-2 specificities, contrary to previous assumptions. Sensitive assays confirmed the presence of K- and D-end H-2 products, not due to differentiation.
Area of Science:
- Immunology
- Cell Biology
- Genetics
Background:
- Murine teratocarcinoma-derived cell line TerC was previously believed to lack major histocompatibility locus H-2 products.
- Understanding H-2 expression in TerC is crucial for studying tumor immunology and cell differentiation.
Purpose of the Study:
- To investigate the expression of major histocompatibility locus H-2 specificities in the TerC cell line.
- To determine if low-level H-2 expression is related to cellular differentiation.
Main Methods:
- Utilized a sensitive cytotoxic assay employing antibody and complement to measure H-2 specificities.
- Assay measured the inhibition of thymidine analogue [125I]5-iodo-2'-deoxyuridine uptake.
- Employed oligospecific antisera and absorption analyses to confirm specificity.
Main Results:
- TerC cells express low but detectable levels of K- and D-end H-2 specificities.
- The presence of both public and private H-2 specificities was confirmed.
- H-2 expression showed minimal variation among TerC clones and did not increase over time in culture.
Conclusions:
- The TerC cell line does express H-2 specificities, albeit at low levels.
- The observed low-level H-2 expression is an intrinsic property of the TerC cell line, not a result of differentiation.