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Mutagens in human urine: test with human peripheral lymphocytes
Mutation Research
|December 1, 1982
Summary
Urine from cancer patients on multi-drug therapy, including cyclophosphamide, significantly increased sister-chromatid exchanges (SCE) in human blood. Healthy individuals' or epileptic patients' urine did not elevate SCE frequencies, indicating potential genotoxicity from cancer treatments.
Area of Science:
- Biochemistry
- Toxicology
- Genetics
Background:
- Sister-chromatid exchanges (SCE) are indicators of DNA damage and genotoxicity.
- Assessing the genotoxic potential of biological fluids like urine is crucial for understanding treatment side effects.
Purpose of the Study:
- To investigate the potential genotoxic effects of human urine on peripheral blood cells.
- To determine if urine from cancer patients undergoing multi-drug therapy induces sister-chromatid exchanges (SCE).
Main Methods:
- Human peripheral blood was exposed to human urine within dialysis bags.
- Post-exposure, blood was cultured with bromodeoxyuridine.
- Metaphase cells were analyzed for sister-chromatid exchange (SCE) frequencies.
Main Results:
- Urine from healthy individuals and epileptic patients on anti-epileptic drugs did not increase SCE frequencies.
- Urine from cancer patients treated with multi-drug therapy, including cyclophosphamide, significantly elevated SCE frequencies.
Conclusions:
- Cyclophosphamide-containing cancer therapy may produce genotoxic substances excreted in urine.
- This study highlights a potential method for assessing the genotoxicity of patient-derived urine samples.