Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altered genetic code in Paramecium mitochondria: possible evolutionary trends

J J Seilhamer, D J Cummings

    Molecular & General Genetics : MGG
    |January 1, 1982
    PubMed
    Summary

    The Paramecium tetraaurelia tRNA trp gene shows a unique anticodon (UCA), altering the universal genetic code. This finding suggests Paramecium mitochondria may represent an early stage in organelle evolution.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Autonomously replicating sequences in young and senescent mitochondrial DNA from Podospora anserina.

    Current genetics·2013
    Same author

    Structural and functional analysis of the origin of replication of mitochondrial DNA from Paramecium aurelia : I. Inverted complements form the terminal loop.

    Current genetics·2013
    Same author

    Mitochondrial DNA from Podospora anserina : IV. The large ribosomal RNA gene contains two long intervening sequences.

    Current genetics·2013
    Same author

    Transcription of a mitochondrial plasmid during senescence in Podospora anserina.

    Current genetics·2013
    Same author

    Gene expression microarray data analysis for toxicology profiling.

    Annals of the New York Academy of Sciences·2000
    Same author

    Large scale gene expression analysis of cholesterol-loaded macrophages.

    The Journal of biological chemistry·2000

    Area of Science:

    • Molecular Biology
    • Genetics
    • Evolutionary Biology

    Background:

    • The genetic code, while largely universal, exhibits variations in certain organelles like mitochondria.
    • Transfer RNA (tRNA) genes are crucial for protein synthesis and can reveal evolutionary relationships.

    Purpose of the Study:

    • To determine the sequence and structure of the tRNA trp gene in Paramecium tetraaurelia.
    • To investigate potential alterations in the genetic code within Paramecium mitochondria.

    Main Methods:

    • Gene sequencing
    • Bioinformatic analysis of tRNA structure and homology

    Main Results:

    • The Paramecium tetraaurelia tRNA trp gene was sequenced and its structure determined.
    • The gene is located 1,500 bp downstream from the 13S rRNA gene.
    • The tRNA trp possesses a UCA anticodon, an alteration from the universal CCA anticodon.
    • Its sequence shows greater homology to bacterial tRNA trp than to other known mitochondrial tRNAs.

    Conclusions:

    • The Paramecium tRNA trp anticodon represents a deviation from the universal genetic code, akin to mitochondrial variations.
    • Paramecium mitochondria share features with other mitochondrial tRNAs but also exhibit unique homology to E. coli.
    • These characteristics suggest Paramecium mitochondria might represent a primitive stage in organelle evolution.

    Related Experiment Videos