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Acantholytic cells in pemphigus. A scanning and transmission electron microscopic study
Abstract:
Scanning electron microscopy (SEM) was performed on oral scrapings from seven patients with pemphigus vulgaris and one with pemphigus vegetans as well as on skin samples from one of those with pemphigus vulgaris. The unstained, fixed, critical-point dried cells were identified with the aid of a light microscope. On the basis of the surface appearance, three main types of acantholytic cells were identified: smooth-surfaced, wrinkled, and microvillous. The acantholytic cells were rounded to ovoid and some showed either a central bulging or a concavity. Small, rounded to ovoid holes or indentations (diameter 0.1-0.3 mumol) were observed on most cell surfaces. The surfaces of acantholytic cells were occasionally occupied by a few micro-organisms. Neutrophilic polymorphonuclear leucocyte/acantholytic cell rosettes were studied by SEM. No gap between acantholytic cell and surrounding leucocytes could be demonstrated. It is postulated that neutrophils may mediate the cytolysis of antibody-coated acantholytic cells. Oral scrapings from one patient with pemphigus vulgaris were collected for transmission electron microscopy (TEM). The acantholytic cells were rounded or ovoid and usually had centrally placed nuclei. Tonofilaments were either randomly distributed or concentrically arranged around the nucleus. In other acantholytic cells there was a halo containing vesicles of varying size around the nucleus. TEM findings suggest that acantholytic cells represent dead or dying cells. Both TEM and SEM findings suggest that when acantholytic cells first separate they may show variable numbers of microvilli, which are probably lost in older acantholytic cells.
Insights
Scanning electron microscopy (SEM) revealed three acantholytic cell types in pemphigus vulgaris. Neutrophils may lyse antibody-coated acantholytic cells, which appear dead or dying.
Area of Science:
- Oral pathology
- Dermatology
- Cell biology
Background:
- Pemphigus vulgaris is an autoimmune blistering disease.
- Acantholytic cells are characteristic of pemphigus.
- Understanding acantholytic cell morphology is crucial for diagnosis.
Purpose of the Study:
- To characterize acantholytic cells using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
- To investigate the interaction between acantholytic cells and neutrophils.
Main Methods:
- SEM and TEM were performed on oral scrapings and skin samples from patients with pemphigus vulgaris and pemphigus vegetans.
- Cells were identified and their surface morphology analyzed.
- Neutrophil-acantholytic cell interactions were examined.
Main Results:
- Three types of acantholytic cells were identified by SEM: smooth-surfaced, wrinkled, and microvillous.
- Acantholytic cells exhibited rounded to ovoid shapes with surface indentations.
- TEM suggested acantholytic cells are dead or dying, with potential microvilli loss over time.
- No gap was observed between acantholytic cells and surrounding neutrophils, suggesting a direct interaction.
Conclusions:
- SEM and TEM provide valuable insights into acantholytic cell morphology in pemphigus.
- Neutrophils may play a role in the cytolysis of antibody-coated acantholytic cells.
- Acantholytic cell surface features, including microvilli, change as cells age.