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The activity of partially purified leukocytic endogenous mediator in endotoxin-resistant C3H/HeJ mice
Abstract:
Partially purified LEM was injected into both the endotoxin-resistant C3H/HeJ and the closely related, but endotoxin-responsive, C3Heb/FeJ strains of mice. Both strains of mice responded to LEM with significant decreases in plasma iron concentration, increased number of peripheral blood neutrophils, increased plasma levels of CSA, and elevations of plasma fibrinogen concentration. Injections of endotoxin produced significant responses in these four determinations in C3Heb/FeJ mice but had no effect in the C3H/HeJ strain. Changes in plasma iron, fibrinogen, CSA, and neutrophils are believed to occur when LEM is produced by an interaction of endotoxin with phagocytic cells.
Insights
Lipid-extracted murine (LEM) affects iron, neutrophils, and fibrinogen in mice. These responses, linked to endotoxin and phagocytic cells, highlight LEM's biological activity.
Area of Science:
- Immunology
- Hematology
- Biochemistry
Background:
- Endotoxin is known to induce significant physiological changes in responsive organisms.
- Lipid-extracted murine (LEM) is a substance whose biological effects, particularly in relation to endotoxin, require further elucidation.
- Understanding the interaction between LEM, endotoxin, and host responses is crucial for comprehending inflammatory processes.
Purpose of the Study:
- To investigate the physiological effects of partially purified LEM in different mouse strains.
- To compare the responses to LEM and endotoxin in endotoxin-responsive versus endotoxin-resistant mice.
- To explore the potential role of phagocytic cells and endotoxin in LEM production and subsequent effects.
Main Methods:
- Injection of partially purified LEM into C3H/HeJ (endotoxin-resistant) and C3Heb/FeJ (endotoxin-responsive) mice.
- Monitoring of plasma iron concentration, peripheral blood neutrophil count, plasma CSA levels, and plasma fibrinogen concentration.
- Administration of endotoxin to both mouse strains to assess differential responses.
Main Results:
- Both mouse strains exhibited decreased plasma iron, increased neutrophils, elevated CSA, and increased fibrinogen following LEM injection.
- Endotoxin administration elicited significant changes in these parameters only in the C3Heb/FeJ (responsive) strain.
- The C3H/HeJ (resistant) strain showed no significant response to endotoxin administration.
Conclusions:
- LEM induces consistent physiological changes regardless of endotoxin responsiveness.
- The observed changes in iron, neutrophils, CSA, and fibrinogen are likely mediated by LEM, potentially produced through endotoxin-phagocytic cell interaction.
- This study suggests LEM plays a role in the host's response to inflammatory stimuli.