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Related Experiment Videos

Transiently elevated apparent lipase by nephelometry

J Yourno, J B Henry

    American Journal of Clinical Pathology
    |July 1, 1978
    PubMed
    Summary
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    Certain patient blood samples exhibit unusual lipase activity, showing normal levels in one test but high levels in another. This non-lipase activity interferes with diagnostic testing, requiring a new detection protocol.

    Area of Science:

    • Clinical chemistry
    • Biochemical diagnostics
    • Enzyme assays

    Background:

    • Lipase assays are crucial for diagnosing pancreatic diseases.
    • Discrepancies in lipase activity measurements can lead to misdiagnosis.
    • Nephelometric assays offer rapid results but can be susceptible to interferences.

    Purpose of the Study:

    • To investigate aberrant lipase activity in patient sera.
    • To characterize the behavior of discordant lipase results in routine assays.
    • To develop a method for identifying non-lipolytic interferences in lipase testing.

    Main Methods:

    • Comparison of titrimetric and nephelometric lipase assays.
    • Analysis of sera with discordant lipase activities.
    • Investigation of reaction kinetics, serial dilution, and heat stability.

    Related Experiment Videos

  • Tracer experiments using "de-emulsification" of substrate.

    • Main Results:

    • Observed normal/low titrimetric lipase with high nephelometric lipase in ~1% of sera.
    • Discordant sera showed nonlinear kinetics, activity decay, and loss upon dilution.
    • No significant free fatty acid release in tracer experiments, indicating non-lipolytic activity.
    • The aberrant activity was partially heat-stable, suggesting a complex interfering substance.

    Conclusions:

    • A non-lipolytic, interfering substance can cause falsely elevated nephelometric lipase activity.
    • This interference mimics high lipase activity but lacks enzymatic function.
    • A practical protocol is proposed to identify these aberrant lipases in clinical laboratories.