Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Ion selectivity and proximal salt reabsorption

C A Berry, D G Warnock, F C Rector

The American Journal of Physiology

|September 11, 1978

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Towards a Field-Portable Real-Time Organic and Elemental Carbon Monitor.

Mining, metallurgy & exploration·2019

Same author

Developmental changes in diaphragm muscle function in the preterm and postnatal lamb.

Pediatric pulmonology·2013

Same author

Membranous glomerulopathy in an adult patient with X-linked agammaglobulinemia receiving intravenous gammaglobulin.

Journal of investigational allergology & clinical immunology·2011

Same author

A gastrin transcript expressed in gastrointestinal cancer cells contains an internal ribosome entry site.

British journal of cancer·2008

Same author

Improving outcomes from acute kidney injury (AKI): Report on an initiative.

The International journal of artificial organs·2007

Same author

Development of the diabetes problem-solving measure for adolescents.

The Diabetes educator·2002

Same journal

Blood coagulation in fish.

The American journal of physiology·2011

Same journal

Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

The American journal of physiology·2010

Same journal

The inactivation of placental toxin by human serum.

The American journal of physiology·2010

Same journal

Adrenal function following ovariectomy in the rat.

The American journal of physiology·2010

Same journal

Capillary permeability; perfusion of frog and guinea pig hind limbs.

The American journal of physiology·2010

Same journal

Evaluation of protective measures against sunburn.

The American journal of physiology·2010

See all related articles

Rabbit proximal convoluted tubules (PCT) exhibit sodium selectivity. Bicarbonate permeability (PHCO3/PCl) varies with active sodium transport, influencing electrical potential and sodium chloride flux in PCT.

Area of Science:

Nephrology
Renal Physiology
Electrophysiology

Background:

The proximal convoluted tubule (PCT) is crucial for renal reabsorption.
Understanding ion transport in PCT is key to kidney function.

Purpose of the Study:

To investigate the relative sodium-to-chloride (PNa/PCl) and bicarbonate-to-chloride (PHCO3/PCl) permeability ratios in superficial (S) and juxtamedullary (JM) rabbit PCT.
To elucidate the influence of active sodium transport on these permeability ratios and the resulting transepithelial electrical potential.

Main Methods:

Isolated perfused rabbit superficial (S) and juxtamedullary (JM) proximal convoluted tubules (PCT).
Electrophysiological techniques to measure ion permeability ratios (PNa/PCl and PHCO3/PCl).
Experiments conducted with and without active sodium transport, and at varying temperatures.

Related Experiment Videos

Main Results:

Most PCT demonstrated sodium selectivity (high PNa/PCl).
PHCO3/PCl was condition-dependent: high and increasing with PNa/PCl during active sodium transport, but approximating 0.5 and independent of PNa/PCl when active transport was inhibited or at 25°C.
Transepithelial electrical potential varied with PNa/PCl and PHCO3/PCl, decreasing from 4.0 mV in S PCT to zero in JM PCT under specific perfusion conditions.

Conclusions:

Active sodium transport significantly impacts PHCO3/PCl by altering paracellular and transcellular current flow.
Anion concentration gradients drive significant sodium chloride diffusion through the paracellular pathway specifically in PCT with low PNa/PCl ratios.