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Myocardial nuclear protein kinases during postnatal development

C J Limas

    The American Journal of Physiology
    |September 1, 1978
    PubMed
    Summary

    As young rats mature, their heart cells’ ability to use DNA for RNA production declines. This decrease in chromatin template activity is linked to reduced nuclear protein kinase activity.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cardiovascular Physiology

    Background:

    • Chromatin template activity in rat myocardial cells decreases postnatally.
    • Myocardial proliferative capacity is restricted during early postnatal development.
    • Nuclear protein kinases are present in myocardial cells.

    Purpose of the Study:

    • To investigate the relationship between declining chromatin template activity and nuclear phosphokinase activity during postnatal development in rat myocardial cells.
    • To determine if changes in protein kinase activity correlate with altered myocardial RNA synthesis.

    Main Methods:

    • Isolation of rat myocardial cells at different postnatal ages.
    • Assay of chromatin template activity.
    • Isolation and characterization of nuclear protein kinases.
    • Measurement of protein kinase activity and substrate specificity.
    • In vitro assessment of myocardial RNA synthesis.

    Main Results:

    • Chromatin template activity declined progressively in the first few weeks of postnatal life.
    • Nuclear protein kinase activity, particularly in fractions III and V, decreased with age.
    • No significant age-related changes were observed in cyclic AMP dependency, substrate specificity, or phosphorylation patterns.
    • Higher protein kinase phosphorylating activity in younger rats correlated with enhanced myocardial RNA synthesis in vitro.

    Conclusions:

    • The decline in chromatin template activity during postnatal development may be partly attributed to decreased nuclear phosphokinase activities.
    • Nuclear phosphokinase activity plays a role in regulating myocardial RNA synthesis and proliferative capacity.

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