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Related Experiment Videos

beta-Lactamase activity in human pus

R E Bryant, A L Rashad, J A Mazza

    The Journal of Infectious Diseases
    |October 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

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    Pus from infections inactivates certain antibiotics like penicillin due to beta-lactamase enzymes. This enzyme activity reduces antibiotic effectiveness, impacting treatment strategies for intra-abdominal abscesses and empyema.

    Area of Science:

    • Microbiology
    • Pharmacology
    • Infectious Diseases

    Background:

    • Polymicrobial infections, such as intra-abdominal abscesses and empyema, present complex treatment challenges.
    • The effectiveness of antibiotics can be compromised by factors within the infection site.

    Purpose of the Study:

    • To investigate the inactivation of common antibiotics in pus from polymicrobial infections.
    • To identify the mechanisms responsible for antibiotic degradation in pus.
    • To assess the impact of pus components on antibiotic bactericidal activity.

    Main Methods:

    • Collected pus specimens from patients with intra-abdominal abscesses or empyema.
    • Analyzed physical and chemical characteristics and enumerated bacterial isolates.
    • Assessed antibiotic inactivation by pus supernatant using various beta-lactam antibiotics.

    Related Experiment Videos

  • Identified the presence and activity of beta-lactamase using nitrocefin, chromatography, and clavulanic acid.
  • Evaluated the effect of pus supernatant on the bactericidal activity of antibiotics against *Bacteroides fragilis* in an in vitro abscess model.

    • Main Results:

    • Pus supernatant from six of twelve specimens rapidly inactivated penicillin, cephalothin, and cefazolin.
    • Carbenicillin and ticarcillin were also degraded by some pus specimens.
    • Cefoxitin, chloramphenicol, and clindamycin remained largely stable.
    • Beta-lactamase activity in pus was confirmed through chemical assays and inhibition studies.
    • Beta-lactamase in pus supernatant reduced the bactericidal activity of carbenicillin against *Bacteroides fragilis*.

    Conclusions:

    • Beta-lactamase, present in pus from polymicrobial infections, inactivates beta-lactam antibiotics.
    • This enzymatic activity can significantly reduce the efficacy of certain antibiotics, like penicillin and cephalosporins.
    • Antibiotics such as cefoxitin, chloramphenicol, and clindamycin are not inactivated by pus beta-lactamase and retain their bactericidal activity.