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Related Experiment Videos

Plasmid transformation in Haemophilus influenzae

W L Albritton, J W Bendler, J K Setlow

    Journal of Bacteriology
    |February 1, 1981
    PubMed
    Summary

    Antibiotic resistance in Haemophilus influenzae is enhanced by specific plasmids. The presence of 30-megadalton plasmids significantly boosts the transformation efficiency of 34-megadalton plasmid deoxyribonucleic acid.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Haemophilus influenzae is a significant human pathogen.
    • Plasmid-mediated antibiotic resistance is a growing concern in H. influenzae.
    • Transformation is a key mechanism for genetic exchange in bacteria.

    Purpose of the Study:

    • To investigate the role of specific plasmids in enhancing transformation efficiency in Haemophilus influenzae.
    • To determine the effect of co-resident plasmids on the uptake of large plasmid deoxyribonucleic acid.

    Main Methods:

    • Purification of 34-megadalton plasmid deoxyribonucleic acid from antibiotic-resistant H. influenzae strains.
    • Transformation assays using competent H. influenzae recipient strains.
    • Comparison of transformation efficiency in recipient strains with and without specific 30-megadalton plasmids.

    Main Results:

    • Purified 34-megadalton plasmid deoxyribonucleic acid was successfully isolated.
    • Transformation efficiency of competent H. influenzae was significantly increased when recipient strains harbored specific 30-megadalton plasmids.
    • The presence of these auxiliary plasmids enhances the uptake of the larger 34-megadalton plasmid.

    Conclusions:

    • Co-resident plasmids can play a crucial role in modulating horizontal gene transfer efficiency in Haemophilus influenzae.
    • The findings highlight a novel mechanism influencing plasmid acquisition and spread in bacterial populations.
    • Understanding these interactions is vital for combating antibiotic resistance.

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