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Cell adhesion molecules: detection with univalent second antibody

W R Springer, S H Barondes

    The Journal of Cell Biology
    |December 1, 1980
    PubMed
    Summary
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    Researchers identified a new method to screen for cell adhesion molecules using intact antibodies. This technique enhances efficiency and reduces antibody usage in cell-cell adhesion studies.

    Area of Science:

    • Cell Biology
    • Immunology
    • Biochemistry

    Background:

    • Cell adhesion molecules (CAMs) are crucial for cell-cell interactions.
    • Identifying CAMs typically involves using univalent antibodies to block adhesion assays.
    • Intact (divalent) antibodies are generally avoided due to potential cell agglutination.

    Purpose of the Study:

    • To develop a more convenient method for identifying cell adhesion molecules.
    • To demonstrate that intact antibodies can be used to block cell-cell adhesion under specific conditions.
    • To facilitate the screening of monoclonal antibodies against potential CAMs.

    Main Methods:

    • Utilized intact rabbit immunoglobulin against Dictyostelium discoideum cell surface molecules.
    • Performed in vitro cell-cell adhesion assays in the presence of univalent goat anti-rabbit antibody.

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  • Optimized experimental conditions to prevent agglutination while maintaining adhesion blocking.

    • Main Results:

    • Intact rabbit antibodies successfully blocked cell-cell adhesion in the presence of univalent goat anti-rabbit antibody.
    • The univalent second antibody effectively inhibited agglutination induced by the intact antibody.
    • The method allowed intact antibody-mediated adhesion blocking without significant interference.

    Conclusions:

    • A novel assay using intact antibodies and a univalent second antibody enables efficient screening for cell adhesion molecules.
    • This method simplifies antibody preparation and reduces antibody consumption compared to traditional techniques.
    • The findings offer a promising approach for identifying novel CAMs and developing related therapeutics.