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Updated: Aug 11, 2026
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
Published on: June 1, 2010
Continuous in vitro culture of human T lymphocytes
Abstract:
A method for continuous culture of human T lymphocytes by stimulation with irradiated lymphocytes, lymphoblastoid cells, and phytohaemagglutinin is described. Cultures can be maintained for at least 9 months. Growth appears to be dependent on a soluble factor released by lymphocytes when they are stimulated with lymphoblastoid cells and phytohaemagglutinin.
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